Journal: BMC Cancer

Article Title: Intratumoral delivery of IL-18 naked DNA induces T-cell activation and Th1 response in a mouse hepatic cancer model

doi: 10.1186/1471-2407-7-87

Figure Lengend Snippet: Immune response is enhanced by a single injection of naked IL-18 plasmid DNA . Each group (consisting of 4 mice) was administered an intratumoral injection of pCEP4 or naked mIL-18 DNA. Spleens of CT26 tumor-bearing mice treated with plasmids were harvested at 7 days after plasmid injection, and cells analyzed by flow cytometry using the corresponding FITC- or PE- or PE-Cy5-labeled antibodies and isotype control.

Article Snippet: The 11 kb mIL-18 DNA expression plasmid vector, pCEP4-mIL18, was constructed using a CMV early enhancer/promoter/EBNA-1-based pCEP4 plasmid vector (Invitrogen, San Diego, CA) with an ampicillin selection gene.

Techniques: Injection, Plasmid Preparation, Flow Cytometry, Labeling

Journal: BMC Cancer

Article Title: Intratumoral delivery of IL-18 naked DNA induces T-cell activation and Th1 response in a mouse hepatic cancer model

doi: 10.1186/1471-2407-7-87

Figure Lengend Snippet: IL-18 gene transfer results in regression of CT26 liver tumors . CT26 cells (1.0 × 10 5 ) were injected into a left lobe of the livers of 6-8-week-old BALB/c mice. pCEP4 or mIL-18 plasmid DNA was injected directly into the tumors on day 7. Tumor growth was measured on days 1, 4, 7, and 14 after plasmid implantation. Data are presented as the mean of tumor weights of 8 mice/group.

Article Snippet: The 11 kb mIL-18 DNA expression plasmid vector, pCEP4-mIL18, was constructed using a CMV early enhancer/promoter/EBNA-1-based pCEP4 plasmid vector (Invitrogen, San Diego, CA) with an ampicillin selection gene.

Techniques: Injection, Plasmid Preparation

Journal: BMC Cancer

Article Title: Intratumoral delivery of IL-18 naked DNA induces T-cell activation and Th1 response in a mouse hepatic cancer model

doi: 10.1186/1471-2407-7-87

Figure Lengend Snippet: Intracellular IFN-γ production by CT26-specific T cells from pmIL-18-treated mice . Splenocytes from CT26-tumor bearing mice treated with three rounds of pCEP4 or mIL-18 were pooled and incubated in medium alone or that containing CT26 lysates. Numbers within the gates in the FACS plots depict the percentage of CD69 + IFN-γ + CD4 + cells. Data are presented from experiments that were repeated at least twice.

Article Snippet: The 11 kb mIL-18 DNA expression plasmid vector, pCEP4-mIL18, was constructed using a CMV early enhancer/promoter/EBNA-1-based pCEP4 plasmid vector (Invitrogen, San Diego, CA) with an ampicillin selection gene.

Techniques: Incubation